Probing the Circumnuclear Stellar Populations of Starburst Galaxies in the Near-infrared

We employ the NASA Infrared Telescope Facility's near-infrared spectrograph SpeX at 0.8-2.4$\mu$m to investigate the spatial distribution of the stellar populations (SPs) in four well known Starburst galaxies: NGC34, NGC1614, NGC3310 and NGC7714. We use the STARLIGHT code updated with the synthetic simple stellar populations models computed by Maraston (2005, M05). Our main results are that the NIR light in the nuclear surroundings of the galaxies is dominated by young/intermediate age SPs ($t \leq 2\times10^9$yr), summing from $\sim$40\% up to 100\% of the light contribution. In the nuclear aperture of two sources (NGC1614 and NGC3310) we detected a predominant old SP component ($t > 2\times10^9$yr), while for NGC34 and NGC7714 the younger component prevails. Furthermore, we found evidence of a circumnuclear star formation ring-like structure and a secondary nucleus in NGC1614, in agreement with previous studies. We also suggest that the merger/interaction experienced by three of the galaxies studied, NGC1614, NGC3310 and NGC7714 can explain the lower metallicity values derived for the young SP component of these sources. In this scenario the fresh unprocessed metal poorer gas from the destroyed/interacting companion galaxy is driven to the centre of the galaxies and mixed with the central region gas, before star formation takes place. In order to deepen our analysis, we performed the same procedure of SP synthesis using Maraston (2011, M11) EPS models. Our results show that the newer and higher resolution M11 models tend to enhance the old/intermediate age SP contribution over the younger ages

The AGNIFS survey: spatially resolved observations of hot molecular and ionised outflows in nearby active galaxies

We present the hot molecular and warm ionised gas kinematics for 33 nearby ($0.001\lesssim z\lesssim0.056$) X-ray selected active galaxies using the H$_2 2.1218 \mu$m and Br$\gamma$ emission lines observed in the K-band with the Gemini Near-Infrared Field Spectrograph (NIFS). The observations cover the inner 0.04$-$2 kpc of each AGN at spatial resolutions of 4$-$250 pc with a velocity resolution of $\sigma_{\rm inst}\approx$20 ${\rm km s^{-1}}$. We find that 31 objects (94 per cent) present a kinematically disturbed region (KDR) seen in ionised gas, while such regions are observed in hot molecular gas for 25 galaxies (76 per cent). We interpret the KDR as being due to outflows with masses of 10$^2-$10$^7$ M$_\odot$ and 10$^0-$10$^4$ M$_\odot$ for the ionised and hot molecular gas, respectively. The ranges of mass-outflow rates ($\dot{M}_{\rm out}$) and kinetic power ($\dot{E}_{\rm K}$) of the outflows are 10$^{-3}-$10$^{1}$ M$_\odot$yr$^{-1}$ and $\sim$10$^{37}$$-$10$^{43}$ erg s$^{-1}$ for the ionised gas outflows, and 10$^{-5}$$-$10$^{-2}$ M$_\odot$ yr$^{-1}$ and 10$^{35}$$-$10$^{39}$ erg s$^{-1}$ for the hot molecular gas outflows. The median coupling efficiency in our sample is $\dot{E}_{K}/L_{\rm bol}\approx1.8\times10^{-3}$ and the estimated momentum fluxes of the outflows suggest they are produced by radiation-pressure in low-density environment, with possible contribution from shocks.Comment: 37 pages, published in MNRAS - A few typos in the text and in the label of Fg 1 were corrected in this versio

Distribution of killer cell immunoglobulin-like receptors genes in the Italian Caucasian population

BACKGROUND: Killer cell immunoglobulin-like receptors (KIRs) are a family of inhibitory and activatory receptors that are expressed by most natural killer (NK) cells. The KIR gene family is polymorphic: genomic diversity is achieved through differences in gene content and allelic polymorphism. The number of KIR loci has been reported to vary among individuals, resulting in different KIR haplotypes. In this study we report the genotypic structure of KIRs in 217 unrelated healthy Italian individuals from 22 immunogenetics laboratories, located in the northern, central and southern regions of Italy. METHODS: Two hundred and seventeen DNA samples were studied by a low resolution PCR-SSP kit designed to identify all KIR genes. RESULTS: All 17 KIR genes were observed in the population with different frequencies than other Caucasian and non-Caucasian populations; framework genes KIR3DL3, KIR3DP1, KIR2DL4 and KIR3DL2 were present in all individuals. Sixty-five different profiles were found in this Italian population study. Haplotype A remains the most prevalent and genotype 1, with a frequency of 28.5%, is the most commonly observed in the Italian population. CONCLUSION: The Italian Caucasian population shows polymorphism of the KIR gene family like other Caucasian and non-Caucasian populations. Although 64 genotypes have been observed, genotype 1 remains the most frequent as already observed in other populations. Such knowledge of the KIR gene distribution in populations is very useful in the study of associations with diseases and in selection of donors for haploidentical bone marrow transplantation

A Bi-Functional Anti-Thrombosis Protein Containing Both Direct-Acting Fibrin(ogen)olytic and Plasminogen-Activating Activities

Direct-acting fibrin(ogen)olytic agents such as plasmin have been proved to contain effective and safety thrombolytic potential. Unfortunately, plasmin is ineffective when administered by the intravenous route because it was neutralized by plasma antiplasmin. Direct-acting fibrin(ogen)olytic agents with resistance against antiplasmin will brighten the prospect of anti-thrombosis. As reported in ‘Compendium of Materia Medica’, the insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years. Currently, we have identified a fibrin(ogen)olytic protein (Eupolytin1) containing both fibrin(ogen)olytic and plasminogen-activating (PA) activities from the beetle, E. sinensis. Objectives: To investigate the role of native and recombinant eupolytin1 in fibrin(ogen)olytic and plasminogen-activating processes. Methods and Results: Using thrombus animal model, eupolytin1 was proved to contain strong and rapid thrombolytic ability and safety in vivo, which are better than that of urokinase. Most importantly, no bleeding complications were appeared even the intravenous dose up to 0.12 µmol/kg body weight (3 times of tested dose which could completely lyse experimental thrombi) in rabbits. It is the first report of thrombolytic agents containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities. Conclusions: The study identified novel thrombolytic agent with prospecting clinical potential because of its bi-functional merits containing both plasmin- and PA-like activities and unique pharmacological kinetics in vivo

Physio-chemical characterization of three-component co-amorphous systems generated by a melt-quench method

The purpose of this work was to evaluate the possibility of creating a ternary co-amorphous system and to determine how the properties of a co-amorphous material are altered by the addition of a selected third component. Piroxicam and indomethacin form a stable co-amorphous with the Tg above room temperature. The third component added was selected based on tendency to crystallise (benzamide, caffeine) or form amorphous (acetaminophen, clotrimazole) on cooling. Generated co-amorphous systems were characterised with TGA, HSM, DSC, FTIR, and XRD. Stable ternary co-amorphous systems were successfully generated, which was confirmed using XRD, DSC and FTIR analysis. In all cases, Tg of the ternary system was lower than the Tg of the binary system, although higher than that of the individual third compound. Upon storage for 4 weeks all created ternary systems showed significantly smaller variation in Tg compared to the binary system. Stable three-component co-amorphous systems can be generated via melt quench method using either a crystalline or amorphous third component. Addition of third component can alter the Tg of co-amorphous system and in all cases created more stable co-amorphous system upon storage. Physical parameters may not be sufficient in predicting the resulting Tg, therefore knowledge of chemical interaction must be brought into equation as well

HLA-class I markers and multiple sclerosis susceptibility in the Italian population

Previous studies reported an association with multiple sclerosis (MS) of distinct HLA-class I markers, namely HLA-A*02, HLA-Cw*05 and MOG-142L. In this work, we tested the association with MS of A*02 and Cw*05 in 1273 Italian MS patients and 1075 matched controls, which were previously analyzed for MOG-142, and explored the relationship among these three markers in modulating MS risk. HLA-A*02 conferred a statistically robust MS protection (odds ratio, OR=0.61; 95% confidence intervals, CI=0.51–0.72, P<10−9), which was independent of DRB1*15 and of any other DRB1* allele and remained similar after accounting for the other two analyzed class I markers. Conversely, the protective effect we previously observed for MOG-142L was secondary to its linkage disequilibrium with A*02. Cw*05 was not associated considering the whole sample, but its presence significantly enhanced the protection in the HLA-A*02-positive group, independently of DRB1: the OR conferred by A*02 in Cw*05-positive individuals (0.22, 95% CI=0.13–0.38) was significantly lower than in Cw*05-negative individuals (0.69, 95% CI=0.58–0.83) with a significant (P=4.94 × 10−5) multiplicative interaction between the two markers. In the absence of A*02, Cw*05 behaved as a risk factor, particularly in combination with DRB1*03 (OR=3.89, P=0.0006), indicating that Cw*05 might be a marker of protective or risk haplotypes, respectively

The AGNIFS survey: distribution and excitation of the hot molecular and ionized gas in the inner kpc of nearby AGN hosts

Galaxie

Unfolding Simulations of Holomyoglobin from Four Mammals: Identification of Intermediates and β-Sheet Formation from Partially Unfolded States

Myoglobin (Mb) is a centrally important, widely studied mammalian protein. While much work has investigated multi-step unfolding of apoMb using acid or denaturant, holomyoglobin unfolding is poorly understood despite its biological relevance. We present here the first systematic unfolding simulations of holoMb and the first comparative study of unfolding of protein orthologs from different species (sperm whale, pig, horse, and harbor seal). We also provide new interpretations of experimental mean molecular ellipticities of myoglobin intermediates, notably correcting for random coil and number of helices in intermediates. The simulated holoproteins at 310 K displayed structures and dynamics in agreement with crystal structures (R g ~1.48-1.51 nm, helicity ~75%). At 400 K, heme was not lost, but some helix loss was observed in pig and horse, suggesting that these helices are less stable in terrestrial species. At 500 K, heme was lost within 1.0-3.7 ns. All four proteins displayed exponentially decaying helix structure within 20 ns. The C- and F-helices were lost quickly in all cases. Heme delayed helix loss, and sperm whale myoglobin exhibited highest retention of heme and D/E helices. Persistence of conformation (RMSD), secondary structure, and ellipticity between 2-11 ns was interpreted as intermediates of holoMb unfolding in all four species. The intermediates resemble those of apoMb notably in A and H helices, but differ substantially in the D-, E- and F-helices, which interact with heme. The identified mechanisms cast light on the role of metal/cofactor in poorly understood holoMb unfolding. We also observed β-sheet formation of several myoglobins at 500 K as seen experimentally, occurring after disruption of helices to a partially unfolded, globally disordered state; heme reduced this tendency and sperm-whale did not display any sheet propensity during the simulations